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        首頁 > 技術(shù)文章 >CEM微波合成用于γ-Lactam的研究

        CEM微波合成用于γ-Lactam的研究

         更新時間:2012-06-29 點擊量:1952

        INTRODUCTION

        One challenge facing peptide synthesis is the -lactam formation of arginine (Figure 1). The formation of -lactam occurs when the internal nitrogen of the guanidine attacks the activated ester, irreversibly forming the lactam ring. This reaction is competitive with peptide-bond formation, greatly reducing the coupling efficiency of arginine.

        Figure 1: γ-Lactam Formation

        The goal of this study was to determine what effect microwave irradiation has on -lactam formation and how to optimize the microwave synthesis of arginine-containing peptides. As a test substrate, we selected the 1992 ABRF study peptide sequence. This sequence is known to suffer from Arg deletion due to -lactam formation.

         
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